Overexpression miR-642 Inhibits Cell Migration and Proliferation by Regulation of KRT19 and Clinical Prognosis Value in Gastric Cancer
نویسندگان
چکیده
Objective: To investigate the expression of miR-642 in gastric cancer and its mechanism proliferation migration. Methods: qRT-PCR was used to detect cancer. Dual luciferase gene reporting system verify direct regulatory effect on KRT19 3' UTR. The mRNA protein levels after transfection were detected by Western blot. IncucyteS long term dynamic cell observation. Kaplan-Meier method analyze relationship between level prognosis patients with Results: tissues lower than that paracancer tissues. fluorescence intensity MGC803 cells significantly decreased co-transfection mimics wild-type KRT19-3 'UTR recombinant vector. transfected siRNA. blot analysis PTEN, Akt mTOR proteins showed different expression. Prognostic survival low had shorter survival. Conclusion: can negatively regulate target KRT19.
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ژورنال
عنوان ژورنال: Journal of biomedical research & environmental sciences
سال: 2023
ISSN: ['2766-2276']
DOI: https://doi.org/10.37871/jbres1713